Two-color nanoscopy of organelles for extended times with HIDE probes

Ling Chu, Jonathan Tyson, Juliana E. Shaw, Felix Rivera-Molina, Anthony J. Koleske, Alanna Schepartz () and Derek K. Toomre ()
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Ling Chu: Yale University School of Medicine
Jonathan Tyson: Yale University
Juliana E. Shaw: Yale University
Felix Rivera-Molina: Yale University School of Medicine
Anthony J. Koleske: Yale University
Alanna Schepartz: Yale University
Derek K. Toomre: Yale University School of Medicine

Nature Communications, 2020, vol. 11, issue 1, 1-8

Abstract: Abstract Performing multi-color nanoscopy for extended times is challenging due to the rapid photobleaching rate of most fluorophores. Here we describe a new fluorophore (Yale-595) and a bio-orthogonal labeling strategy that enables two-color super-resolution (STED) and 3D confocal imaging of two organelles simultaneously for extended times using high-density environmentally sensitive (HIDE) probes. Because HIDE probes are small, cell-permeant molecules, they can visualize dual organelle dynamics in hard-to-transfect cell lines by super-resolution for over an order of magnitude longer than with tagged proteins. The extended time domain possible using these tools reveals dynamic nanoscale targeting between different organelles.

Date: 2020
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17859-1

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DOI: 10.1038/s41467-020-17859-1

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