Single-cell transcriptomes of pancreatic preinvasive lesions and cancer reveal acinar metaplastic cells’ heterogeneity

Yehuda Schlesinger, Oshri Yosefov-Levi, Dror Kolodkin-Gal, Roy Zvi Granit, Luriano Peters, Rachel Kalifa, Lei Xia, Abdelmajeed Nasereddin, Idit Shiff, Osher Amran, Yuval Nevo, Sharona Elgavish, Karine Atlan, Gideon Zamir () and Oren Parnas ()
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Yehuda Schlesinger: Hebrew University–Hadassah Medical School
Oshri Yosefov-Levi: Hebrew University–Hadassah Medical School
Dror Kolodkin-Gal: Hadassah–Hebrew University Medical Center
Roy Zvi Granit: Hebrew University–Hadassah Medical School
Luriano Peters: Hebrew University–Hadassah Medical School
Rachel Kalifa: Hadassah–Hebrew University Medical Center
Lei Xia: Hebrew University–Hadassah Medical School
Abdelmajeed Nasereddin: The Hebrew University of Jerusalem
Idit Shiff: The Hebrew University of Jerusalem
Osher Amran: Hebrew University–Hadassah Medical School
Yuval Nevo: Info-CORE, Bioinformatics Unit of the I-CORE at the Hebrew University of Jerusalem and Hadassah Medical Center
Sharona Elgavish: Info-CORE, Bioinformatics Unit of the I-CORE at the Hebrew University of Jerusalem and Hadassah Medical Center
Karine Atlan: Hadassah–Hebrew University Medical Center
Gideon Zamir: Hadassah–Hebrew University Medical Center
Oren Parnas: Hebrew University–Hadassah Medical School

Nature Communications, 2020, vol. 11, issue 1, 1-18

Abstract: Abstract Acinar metaplasia is an initial step in a series of events that can lead to pancreatic cancer. Here we perform single-cell RNA-sequencing of mouse pancreas during the progression from preinvasive stages to tumor formation. Using a reporter gene, we identify metaplastic cells that originated from acinar cells and express two transcription factors, Onecut2 and Foxq1. Further analyses of metaplastic acinar cell heterogeneity define six acinar metaplastic cell types and states, including stomach-specific cell types. Localization of metaplastic cell types and mixture of different metaplastic cell types in the same pre-malignant lesion is shown. Finally, single-cell transcriptome analyses of tumor-associated stromal, immune, endothelial and fibroblast cells identify signals that may support tumor development, as well as the recruitment and education of immune cells. Our findings are consistent with the early, premalignant formation of an immunosuppressive environment mediated by interactions between acinar metaplastic cells and other cells in the microenvironment.

Date: 2020
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DOI: 10.1038/s41467-020-18207-z

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