Siah2 integrates mitogenic and extracellular matrix signals linking neuronal progenitor ciliogenesis with germinal zone occupancy

Taren Ong, Niraj Trivedi, Randall Wakefield, Sharon Frase and David J. Solecki ()
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Taren Ong: St. Jude Children’s Research Hospital
Niraj Trivedi: St. Jude Children’s Research Hospital
Randall Wakefield: Cell and Tissue Imaging Center-EM, St. Jude Children’s Research Hospital
Sharon Frase: St. Jude Children’s Research Hospital
David J. Solecki: St. Jude Children’s Research Hospital

Nature Communications, 2020, vol. 11, issue 1, 1-16

Abstract: Abstract Evidence is lacking as to how developing neurons integrate mitogenic signals with microenvironment cues to control proliferation and differentiation. We determine that the Siah2 E3 ubiquitin ligase functions in a coincidence detection circuit linking responses to the Shh mitogen and the extracellular matrix to control cerebellar granule neurons (CGN) GZ occupancy. We show that Shh signaling maintains Siah2 expression in CGN progenitors (GNPs) in a Ras/Mapk-dependent manner. Siah2 supports ciliogenesis in a feed-forward fashion by restraining cilium disassembly. Efforts to identify sources of the Ras/Mapk signaling led us to discover that GNPs respond to laminin, but not vitronectin, in the GZ microenvironment via integrin β1 receptors, which engages the Ras/Mapk cascade with Shh, and that this niche interaction is essential for promoting GNP ciliogenesis. As GNPs leave the GZ, differentiation is driven by changing extracellular cues that diminish Siah2-activity leading to primary cilia shortening and attenuation of the mitogenic response.

Date: 2020
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DOI: 10.1038/s41467-020-19063-7

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