An enhanced isothermal amplification assay for viral detection

Jason Qian, Sarah A. Boswell, Christopher Chidley, Zhi-xiang Lu, Mary E. Pettit, Benjamin L. Gaudio, Jesse M. Fajnzylber, Ryan T. Ingram, Rebecca H. Ward, Jonathan Z. Li and Michael Springer ()
Additional contact information
Jason Qian: Harvard Medical School
Sarah A. Boswell: Harvard Medical School
Christopher Chidley: Harvard Medical School
Zhi-xiang Lu: Harvard Medical School
Mary E. Pettit: Harvard Medical School
Benjamin L. Gaudio: Harvard Medical School
Jesse M. Fajnzylber: Harvard Medical School
Ryan T. Ingram: Harvard Medical School
Rebecca H. Ward: Harvard Medical School
Jonathan Z. Li: Harvard Medical School
Michael Springer: Harvard Medical School

Nature Communications, 2020, vol. 11, issue 1, 1-10

Abstract: Abstract Rapid, inexpensive, robust diagnostics are essential to control the spread of infectious diseases. Current state of the art diagnostics are highly sensitive and specific, but slow, and require expensive equipment. Here we report the development of a molecular diagnostic test for SARS-CoV-2 based on an enhanced recombinase polymerase amplification (eRPA) reaction. eRPA has a detection limit on patient samples down to 5 viral copies, requires minimal instrumentation, and is highly scalable and inexpensive. eRPA does not cross-react with other common coronaviruses, does not require RNA purification, and takes ~45 min from sample collection to results. eRPA represents a first step toward at-home SARS-CoV-2 detection and can be adapted to future viruses within days of genomic sequence availability.

Date: 2020
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-020-19258-y Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19258-y

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-020-19258-y

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().