Docking sites inside Cas9 for adenine base editing diversification and RNA off-target elimination

Shuo Li, Bo Yuan, Jixin Cao, Jingqi Chen, Jinlong Chen, Jiayi Qiu, Xing-Ming Zhao, Xiaolin Wang (), Zilong Qiu () and Tian-Lin Cheng ()
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Shuo Li: Fudan University
Bo Yuan: Chinese Academy of Sciences
Jixin Cao: Fudan University
Jingqi Chen: Fudan University
Jinlong Chen: Fudan University
Jiayi Qiu: Fudan University
Xing-Ming Zhao: Fudan University
Xiaolin Wang: Fudan University
Zilong Qiu: Chinese Academy of Sciences
Tian-Lin Cheng: Fudan University

Nature Communications, 2020, vol. 11, issue 1, 1-11

Abstract: Abstract Base editing tools with diversified editing scopes and minimized RNA off-target activities are required for broad applications. Nevertheless, current Streptococcus pyogenes Cas9 (SpCas9)-based adenine base editors (ABEs) with minimized RNA off-target activities display constrained editing scopes with efficient editing activities at positions 4-8. Here, functional ABE variants with diversified editing scopes and reduced RNA off-target activities are identified using domain insertion profiling inside SpCas9 and with different combinations of TadA variants. Engineered ABE variants in this study display narrowed, expanded or shifted editing scopes with efficient editing activities across protospacer positions 2-16. And when combined with deaminase engineering, the RNA off-target activities of engineered ABE variants are further minimized. Thus, domain insertion profiling provides a framework to improve and expand ABE toolkits, and its combination with other strategies for ABE engineering deserves comprehensive explorations in the future.

Date: 2020
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DOI: 10.1038/s41467-020-19730-9

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