SVIP is a molecular determinant of lysosomal dynamic stability, neurodegeneration and lifespan

Alyssa E. Johnson, Brian O. Orr, Richard D. Fetter, Armen J. Moughamian, Logan A. Primeaux, Ethan G. Geier, Jennifer S. Yokoyama, Bruce L. Miller and Graeme W. Davis ()
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Alyssa E. Johnson: University of California, San Francisco
Brian O. Orr: University of California, San Francisco
Richard D. Fetter: University of California, San Francisco
Armen J. Moughamian: University of California, San Francisco
Logan A. Primeaux: Louisiana State University
Ethan G. Geier: Louisiana State University
Jennifer S. Yokoyama: University of California, San Francisco
Bruce L. Miller: University of California, San Francisco
Graeme W. Davis: University of California, San Francisco

Nature Communications, 2021, vol. 12, issue 1, 1-17

Abstract: Abstract Missense mutations in Valosin-Containing Protein (VCP) are linked to diverse degenerative diseases including IBMPFD, amyotrophic lateral sclerosis (ALS), muscular dystrophy and Parkinson’s disease. Here, we characterize a VCP-binding co-factor (SVIP) that specifically recruits VCP to lysosomes. SVIP is essential for lysosomal dynamic stability and autophagosomal–lysosomal fusion. SVIP mutations cause muscle wasting and neuromuscular degeneration while muscle-specific SVIP over-expression increases lysosomal abundance and is sufficient to extend lifespan in a context, stress-dependent manner. We also establish multiple links between SVIP and VCP-dependent disease in our Drosophila model system. A biochemical screen identifies a disease-causing VCP mutation that prevents SVIP binding. Conversely, over-expression of an SVIP mutation that prevents VCP binding is deleterious. Finally, we identify a human SVIP mutation and confirm the pathogenicity of this mutation in our Drosophila model. We propose a model for VCP disease based on the differential, co-factor-dependent recruitment of VCP to intracellular organelles.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-020-20796-8

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DOI: 10.1038/s41467-020-20796-8

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