Cryo-EM structures of engineered active bc1-cbb3 type CIII2CIV super-complexes and electronic communication between the complexes

Stefan Steimle, Trevor van Eeuwen, Yavuz Ozturk, Hee Jong Kim, Merav Braitbard, Nur Selamoglu, Benjamin A. Garcia, Dina Schneidman-Duhovny, Kenji Murakami () and Fevzi Daldal ()
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Stefan Steimle: University of Pennsylvania
Trevor van Eeuwen: Perelman School of Medicine, University of Pennsylvania
Yavuz Ozturk: University of Pennsylvania
Hee Jong Kim: Perelman School of Medicine, University of Pennsylvania
Merav Braitbard: Institute of Life Sciences, The Hebrew University of Jerusalem
Nur Selamoglu: University of Pennsylvania
Benjamin A. Garcia: Perelman School of Medicine, University of Pennsylvania
Dina Schneidman-Duhovny: Institute of Life Sciences, The Hebrew University of Jerusalem
Kenji Murakami: Perelman School of Medicine, University of Pennsylvania
Fevzi Daldal: University of Pennsylvania

Nature Communications, 2021, vol. 12, issue 1, 1-14

Abstract: Abstract Respiratory electron transport complexes are organized as individual entities or combined as large supercomplexes (SC). Gram-negative bacteria deploy a mitochondrial-like cytochrome (cyt) bc1 (Complex III, CIII2), and may have specific cbb3-type cyt c oxidases (Complex IV, CIV) instead of the canonical aa3-type CIV. Electron transfer between these complexes is mediated by soluble (c2) and membrane-anchored (cy) cyts. Here, we report the structure of an engineered bc1-cbb3 type SC (CIII2CIV, 5.2 Å resolution) and three conformers of native CIII2 (3.3 Å resolution). The SC is active in vivo and in vitro, contains all catalytic subunits and cofactors, and two extra transmembrane helices attributed to cyt cy and the assembly factor CcoH. The cyt cy is integral to SC, its cyt domain is mobile and it conveys electrons to CIV differently than cyt c2. The successful production of a native-like functional SC and determination of its structure illustrate the characteristics of membrane-confined and membrane-external respiratory electron transport pathways in Gram-negative bacteria.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-21051-4

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DOI: 10.1038/s41467-021-21051-4

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