Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors

Plinio S. Vieira, Isabela M. Bonfim, Evandro A. Araujo, Ricardo R. Melo, Augusto R. Lima, Melissa R. Fessel, Douglas A. A. Paixão, Gabriela F. Persinoti, Silvana A. Rocco, Tatiani B. Lima, Renan A. S. Pirolla, Mariana A. B. Morais, Jessica B. L. Correa, Leticia M. Zanphorlin, Jose A. Diogo, Evandro A. Lima, Adriana Grandis, Marcos S. Buckeridge, Fabio C. Gozzo, Celso E. Benedetti, Igor Polikarpov, Priscila O. Giuseppe () and Mario T. Murakami ()
Additional contact information
Plinio S. Vieira: Brazilian Center for Research in Energy and Materials (CNPEM)
Isabela M. Bonfim: Brazilian Center for Research in Energy and Materials (CNPEM)
Evandro A. Araujo: Brazilian Center for Research in Energy and Materials (CNPEM)
Ricardo R. Melo: Brazilian Center for Research in Energy and Materials (CNPEM)
Augusto R. Lima: Brazilian Center for Research in Energy and Materials (CNPEM)
Melissa R. Fessel: Butantan Foundation
Douglas A. A. Paixão: Brazilian Center for Research in Energy and Materials (CNPEM)
Gabriela F. Persinoti: Brazilian Center for Research in Energy and Materials (CNPEM)
Silvana A. Rocco: Brazilian Center for Research in Energy and Materials (CNPEM)
Tatiani B. Lima: Brazilian Center for Research in Energy and Materials (CNPEM)
Renan A. S. Pirolla: Brazilian Center for Research in Energy and Materials (CNPEM)
Mariana A. B. Morais: Brazilian Center for Research in Energy and Materials (CNPEM)
Jessica B. L. Correa: Brazilian Center for Research in Energy and Materials (CNPEM)
Leticia M. Zanphorlin: Brazilian Center for Research in Energy and Materials (CNPEM)
Jose A. Diogo: Brazilian Center for Research in Energy and Materials (CNPEM)
Evandro A. Lima: Brazilian Center for Research in Energy and Materials (CNPEM)
Adriana Grandis: University of São Paulo
Marcos S. Buckeridge: University of São Paulo
Fabio C. Gozzo: University of Campinas
Celso E. Benedetti: Brazilian Center for Research in Energy and Materials (CNPEM)
Igor Polikarpov: University of São Paulo
Priscila O. Giuseppe: Brazilian Center for Research in Energy and Materials (CNPEM)
Mario T. Murakami: Brazilian Center for Research in Energy and Materials (CNPEM)

Nature Communications, 2021, vol. 12, issue 1, 1-15

Abstract: Abstract Xyloglucans are highly substituted and recalcitrant polysaccharides found in the primary cell walls of vascular plants, acting as a barrier against pathogens. Here, we reveal that the diverse and economically relevant Xanthomonas bacteria are endowed with a xyloglucan depolymerization machinery that is linked to pathogenesis. Using the citrus canker pathogen as a model organism, we show that this system encompasses distinctive glycoside hydrolases, a modular xyloglucan acetylesterase and specific membrane transporters, demonstrating that plant-associated bacteria employ distinct molecular strategies from commensal gut bacteria to cope with xyloglucans. Notably, the sugars released by this system elicit the expression of several key virulence factors, including the type III secretion system, a membrane-embedded apparatus to deliver effector proteins into the host cells. Together, these findings shed light on the molecular mechanisms underpinning the intricate enzymatic machinery of Xanthomonas to depolymerize xyloglucans and uncover a role for this system in signaling pathways driving pathogenesis.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24277-4

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DOI: 10.1038/s41467-021-24277-4

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