Protein identification by nanopore peptide profiling

Florian Leonardus Rudolfus Lucas, Roderick Corstiaan Abraham Versloot, Liubov Yakovlieva, Marthe T. C. Walvoort and Giovanni Maglia ()
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Florian Leonardus Rudolfus Lucas: University of Groningen
Roderick Corstiaan Abraham Versloot: University of Groningen
Liubov Yakovlieva: University of Groningen
Marthe T. C. Walvoort: University of Groningen
Giovanni Maglia: University of Groningen

Nature Communications, 2021, vol. 12, issue 1, 1-9

Abstract: Abstract Nanopores are single-molecule sensors used in nucleic acid analysis, whereas their applicability towards full protein identification has yet to be demonstrated. Here, we show that an engineered Fragaceatoxin C nanopore is capable of identifying individual proteins by measuring peptide spectra that are produced from hydrolyzed proteins. Using model proteins, we show that the spectra resulting from nanopore experiments and mass spectrometry share similar profiles, hence allowing protein fingerprinting. The intensity of individual peaks provides information on the concentration of individual peptides, indicating that this approach is quantitative. Our work shows the potential of a low-cost, portable nanopore-based analyzer for protein identification.

Date: 2021
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DOI: 10.1038/s41467-021-26046-9

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