Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast

Mariam Jaafar, Julia Contreras, Carine Dominique, Sara Martín-Villanueva, Régine Capeyrou, Patrice Vitali, Olga Rodríguez-Galán, Carmen Velasco, Odile Humbert, Nicholas J. Watkins, Eduardo Villalobo, Katherine E. Bohnsack, Markus T. Bohnsack, Yves Henry, Raghida Abou Merhi, Jesús de la Cruz and Anthony K. Henras ()
Additional contact information
Mariam Jaafar: Université de Toulouse, CNRS, UPS
Julia Contreras: Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
Carine Dominique: Université de Toulouse, CNRS, UPS
Sara Martín-Villanueva: Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
Régine Capeyrou: Université de Toulouse, CNRS, UPS
Patrice Vitali: Université de Toulouse, CNRS, UPS
Olga Rodríguez-Galán: Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
Carmen Velasco: Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
Odile Humbert: Université de Toulouse, CNRS, UPS
Nicholas J. Watkins: Newcastle University
Eduardo Villalobo: Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
Katherine E. Bohnsack: University Medical Centre Göttingen
Markus T. Bohnsack: University Medical Centre Göttingen
Yves Henry: Université de Toulouse, CNRS, UPS
Raghida Abou Merhi: Lebanese University
Jesús de la Cruz: Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla
Anthony K. Henras: Université de Toulouse, CNRS, UPS

Nature Communications, 2021, vol. 12, issue 1, 1-17

Abstract: Abstract Synthesis of eukaryotic ribosomes involves the assembly and maturation of precursor particles (pre-ribosomal particles) containing ribosomal RNA (rRNA) precursors, ribosomal proteins (RPs) and a plethora of assembly factors (AFs). Formation of the earliest precursors of the 60S ribosomal subunit (pre-60S r-particle) is among the least understood stages of ribosome biogenesis. It involves the Npa1 complex, a protein module suggested to play a key role in the early structuring of the pre-rRNA. Npa1 displays genetic interactions with the DExD-box protein Dbp7 and interacts physically with the snR190 box C/D snoRNA. We show here that snR190 functions as a snoRNA chaperone, which likely cooperates with the Npa1 complex to initiate compaction of the pre-rRNA in early pre-60S r-particles. We further show that Dbp7 regulates the dynamic base-pairing between snR190 and the pre-rRNA within the earliest pre-60S r-particles, thereby participating in structuring the peptidyl transferase center (PTC) of the large ribosomal subunit.

Date: 2021
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DOI: 10.1038/s41467-021-26207-w

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