Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry

Che-Hang Yu, Jeffrey N. Stirman, Yiyi Yu, Riichiro Hira and Spencer L. Smith ()
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Che-Hang Yu: University of California
Jeffrey N. Stirman: LifeCanvas Technologies
Yiyi Yu: University of California
Riichiro Hira: University of California
Spencer L. Smith: University of California

Nature Communications, 2021, vol. 12, issue 1, 1-8

Abstract: Abstract Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p). Combining optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view of ~25 mm2, encompassing distances up to 7 mm, with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain.

Date: 2021
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DOI: 10.1038/s41467-021-26736-4

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