Colocalized, bidirectional optogenetic modulations in freely behaving mice with a wireless dual-color optoelectronic probe

Lizhu Li, Lihui Lu, Yuqi Ren, Guo Tang, Yu Zhao, Xue Cai, Zhao Shi, He Ding, Changbo Liu, Dali Cheng, Yang Xie, Huachun Wang, Xin Fu, Lan Yin, Minmin Luo () and Xing Sheng ()
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Lizhu Li: Tsinghua University
Lihui Lu: Chinese Institute for Brain Research
Yuqi Ren: Chinese Institute for Brain Research
Guo Tang: Tsinghua University
Yu Zhao: Tsinghua University
Xue Cai: Tsinghua University
Zhao Shi: Tsinghua University
He Ding: Beijing Institute of Technology
Changbo Liu: Beihang University
Dali Cheng: Tsinghua University
Yang Xie: Tsinghua University
Huachun Wang: Tsinghua University
Xin Fu: Tsinghua University
Lan Yin: Tsinghua University
Minmin Luo: Chinese Institute for Brain Research
Xing Sheng: Tsinghua University

Nature Communications, 2022, vol. 13, issue 1, 1-14

Abstract: Abstract Optogenetic methods provide efficient cell-specific modulations, and the ability of simultaneous neural activation and inhibition in the same brain region of freely moving animals is highly desirable. Here we report bidirectional neuronal activity manipulation accomplished by a wireless, dual-color optogenetic probe in synergy with the co-expression of two spectrally distinct opsins (ChrimsonR and stGtACR2) in a rodent model. The flexible probe comprises vertically assembled, thin-film microscale light-emitting diodes with a lateral dimension of 125 × 180 µm2, showing colocalized red and blue emissions and enabling chronic in vivo operations with desirable biocompatibilities. Red or blue irradiations deterministically evoke or silence neurons co-expressing the two opsins. The probe interferes with dopaminergic neurons in the ventral tegmental area of mice, increasing or decreasing dopamine levels. Such bidirectional regulations further generate rewarding and aversive behaviors and interrogate social interactions among multiple mice. These technologies create numerous opportunities and implications for brain research.

Date: 2022
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DOI: 10.1038/s41467-022-28539-7

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