Genotyping-by-sequencing-based identification of Arabidopsis pattern recognition receptor RLP32 recognizing proteobacterial translation initiation factor IF1

Li Fan, Katja Fröhlich, Eric Melzer, Rory N. Pruitt, Isabell Albert, Lisha Zhang, Anna Joe, Chenlei Hua, Yanyue Song, Markus Albert, Sang-Tae Kim, Detlef Weigel, Cyril Zipfel, Eunyoung Chae (), Andrea A. Gust () and Thorsten Nürnberger ()
Additional contact information
Li Fan: University of Tübingen
Katja Fröhlich: University of Tübingen
Eric Melzer: University of Tübingen
Rory N. Pruitt: University of Tübingen
Isabell Albert: University of Tübingen
Lisha Zhang: University of Tübingen
Anna Joe: University of Tübingen
Chenlei Hua: University of Tübingen
Yanyue Song: University of Tübingen
Markus Albert: University of Tübingen
Sang-Tae Kim: Max Planck Institute for Developmental Biology
Detlef Weigel: Max Planck Institute for Developmental Biology
Cyril Zipfel: University of Zürich
Eunyoung Chae: Max Planck Institute for Developmental Biology
Andrea A. Gust: University of Tübingen
Thorsten Nürnberger: University of Tübingen

Nature Communications, 2022, vol. 13, issue 1, 1-13

Abstract: Abstract Activation of plant pattern-triggered immunity (PTI) relies on the recognition of microbe-derived structures, termed patterns, through plant-encoded surface-resident pattern recognition receptors (PRRs). We show that proteobacterial translation initiation factor 1 (IF1) triggers PTI in Arabidopsis thaliana and related Brassicaceae species. Unlike for most other immunogenic patterns, IF1 elicitor activity cannot be assigned to a small peptide epitope, suggesting that tertiary fold features are required for IF1 receptor activation. We have deployed natural variation in IF1 sensitivity to identify Arabidopsis leucine-rich repeat (LRR) receptor-like protein 32 (RLP32) as IF1 receptor using a restriction site-associated DNA sequencing approach. RLP32 confers IF1 sensitivity to rlp32 mutants, IF1-insensitive Arabidopsis accessions and IF1-insensitive Nicotiana benthamiana, binds IF1 specifically and forms complexes with LRR receptor kinases SOBIR1 and BAK1 to mediate signaling. Similar to other PRRs, RLP32 confers resistance to Pseudomonas syringae, highlighting an unexpectedly complex array of bacterial pattern sensors within a single plant species.

Date: 2022
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DOI: 10.1038/s41467-022-28887-4

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