Extra kinetic dimensions for label discrimination

Raja Chouket, Agnès Pellissier-Tanon, Aliénor Lahlou, Ruikang Zhang, Diana Kim, Marie-Aude Plamont, Mingshu Zhang, Xi Zhang, Pingyong Xu, Nicolas Desprat, Dominique Bourgeois, Agathe Espagne, Annie Lemarchand (), Thomas Le Saux () and Ludovic Jullien ()
Additional contact information
Raja Chouket: PSL University, Sorbonne Université, CNRS
Agnès Pellissier-Tanon: PSL University, Sorbonne Université, CNRS
Aliénor Lahlou: PSL University, Sorbonne Université, CNRS
Ruikang Zhang: PSL University, Sorbonne Université, CNRS
Diana Kim: PSL University, Sorbonne Université, CNRS
Marie-Aude Plamont: PSL University, Sorbonne Université, CNRS
Mingshu Zhang: Chinese Academy of Sciences
Xi Zhang: Chinese Academy of Sciences
Pingyong Xu: Chinese Academy of Sciences
Nicolas Desprat: PSL University, CNRS, Sorbonne Université, Université de Paris
Dominique Bourgeois: Univ. Grenoble Alpes, CNRS, CEA, IBS
Agathe Espagne: PSL University, Sorbonne Université, CNRS
Annie Lemarchand: LPTMC, Sorbonne Université, CNRS
Thomas Le Saux: PSL University, Sorbonne Université, CNRS
Ludovic Jullien: PSL University, Sorbonne Université, CNRS

Nature Communications, 2022, vol. 13, issue 1, 1-8

Abstract: Abstract Due to its sensitivity and versatility, fluorescence is widely used to detect specifically labeled biomolecules. However, fluorescence is currently limited by label discrimination, which suffers from the broad full width of the absorption/emission bands and the narrow lifetime distribution of the bright fluorophores. We overcome this limitation by introducing extra kinetic dimensions through illuminations of reversibly photoswitchable fluorophores (RSFs) at different light intensities. In this expanded space, each RSF is characterized by a chromatic aberration-free kinetic fingerprint of photochemical reactivity, which can be recovered with limited hardware, excellent photon budget, and minimal data processing. This fingerprint was used to identify and discriminate up to 20 among 22 spectrally similar reversibly photoswitchable fluorescent proteins (RSFPs) in less than 1s. This strategy opens promising perspectives for expanding the multiplexing capabilities of fluorescence imaging.

Date: 2022
References: View references in EconPapers View complete reference list from CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-022-29172-0 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29172-0

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-022-29172-0

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().