Brain-wide projection reconstruction of single functionally defined neurons

Meng Wang, Ke Liu, Junxia Pan, Jialin Li, Pei Sun, Yongsheng Zhang, Longhui Li, Wenyan Guo, Qianqian Xin, Zhikai Zhao, Yurong Liu, Zhenqiao Zhou, Jing Lyu, Ting Zheng, Yunyun Han, Chunqing Zhang, Xiang Liao (), Shaoqun Zeng (), Hongbo Jia () and Xiaowei Chen ()
Additional contact information
Meng Wang: Third Military Medical University
Ke Liu: Third Military Medical University
Junxia Pan: Third Military Medical University
Jialin Li: Chongqing University
Pei Sun: Third Military Medical University
Yongsheng Zhang: Huazhong University of Science and Technology
Longhui Li: Chongqing University
Wenyan Guo: Huazhong University of Science and Technology
Qianqian Xin: Third Military Medical University
Zhikai Zhao: Third Military Medical University
Yurong Liu: Huazhong University of Science and Technology
Zhenqiao Zhou: Chinese Academy of Sciences
Jing Lyu: Chinese Academy of Sciences
Ting Zheng: Huazhong University of Science and Technology
Yunyun Han: School of Basic Medicine and Tongji Medical College, Huazhong University of Science & Technology
Chunqing Zhang: Third Military Medical University
Xiang Liao: Chongqing University
Shaoqun Zeng: Huazhong University of Science and Technology
Hongbo Jia: Guangxi University
Xiaowei Chen: Third Military Medical University

Nature Communications, 2022, vol. 13, issue 1, 1-14

Abstract: Abstract Reconstructing axonal projections of single neurons at the whole-brain level is currently a converging goal of the neuroscience community that is fundamental for understanding the logic of information flow in the brain. Thousands of single neurons from different brain regions have recently been morphologically reconstructed, but the corresponding physiological functional features of these reconstructed neurons are unclear. By combining two-photon Ca2+ imaging with targeted single-cell plasmid electroporation, we reconstruct the brain-wide morphologies of single neurons that are defined by a sound-evoked response map in the auditory cortices (AUDs) of awake mice. Long-range interhemispheric projections can be reliably labelled via co-injection with an adeno-associated virus, which enables enhanced expression of indicator protein in the targeted neurons. Here we show that this method avoids the randomness and ambiguity of conventional methods of neuronal morphological reconstruction, offering an avenue for developing a precise one-to-one map of neuronal projection patterns and physiological functional features.

Date: 2022
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DOI: 10.1038/s41467-022-29229-0

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