Cryo-EM structure of an amyloid fibril formed by full-length human SOD1 reveals its conformational conversion

Li-Qiang Wang, Yeyang Ma, Han-Ye Yuan, Kun Zhao, Mu-Ya Zhang, Qiang Wang, Xi Huang, Wen-Chang Xu, Bin Dai, Jie Chen, Dan Li, Delin Zhang, Zhengzhi Wang, Liangyu Zou, Ping Yin, Cong Liu () and Yi Liang ()
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Li-Qiang Wang: Wuhan University
Yeyang Ma: Chinese Academy of Sciences
Han-Ye Yuan: Wuhan University
Kun Zhao: Chinese Academy of Sciences
Mu-Ya Zhang: Wuhan University
Qiang Wang: Huazhong Agricultural University
Xi Huang: Jinan University (Shenzhen People’s Hospital)
Wen-Chang Xu: Wuhan University
Bin Dai: Shanghai Jiao Tong University
Jie Chen: Wuhan University
Dan Li: Shanghai Jiao Tong University
Delin Zhang: Huazhong Agricultural University
Zhengzhi Wang: Wuhan University
Liangyu Zou: Jinan University (Shenzhen People’s Hospital)
Ping Yin: Huazhong Agricultural University
Cong Liu: Chinese Academy of Sciences
Yi Liang: Wuhan University

Nature Communications, 2022, vol. 13, issue 1, 1-10

Abstract: Abstract Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease. Misfolded Cu, Zn-superoxide dismutase (SOD1) has been linked to both familial and sporadic ALS. SOD1 fibrils formed in vitro share toxic properties with ALS inclusions. Here we produced cytotoxic amyloid fibrils from full-length apo human SOD1 under reducing conditions and determined the atomic structure using cryo-EM. The SOD1 fibril consists of a single protofilament with a left-handed helix. The fibril core exhibits a serpentine fold comprising N-terminal segment (residues 3–55) and C-terminal segment (residues 86–153) with an intrinsic disordered segment. The two segments are zipped up by three salt bridge pairs. By comparison with the structure of apo SOD1 dimer, we propose that eight β-strands (to form a β-barrel) and one α-helix in the subunit of apo SOD1 convert into thirteen β-strands stabilized by five hydrophobic cavities in the SOD1 fibril. Our data provide insights into how SOD1 converts between structurally and functionally distinct states.

Date: 2022
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DOI: 10.1038/s41467-022-31240-4

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