Ciliary transition zone proteins coordinate ciliary protein composition and ectosome shedding

Liang Wang (), Xin Wen, Zhengmao Wang, Zaisheng Lin, Chunhong Li, Huilin Zhou, Huimin Yu, Yuhan Li, Yifei Cheng, Yuling Chen, Geer Lou, Junmin Pan and Muqing Cao ()
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Liang Wang: Jiangsu Normal University
Xin Wen: Jiangsu Normal University
Zhengmao Wang: Qingdao National Laboratory for Marine Science and Technology
Zaisheng Lin: Shanghai Jiao Tong University School of Medicine
Chunhong Li: Jiangsu Normal University
Huilin Zhou: Jiangsu Normal University
Huimin Yu: Jiangsu Normal University
Yuhan Li: Jiangsu Normal University
Yifei Cheng: Jiangsu Normal University
Yuling Chen: Tsinghua University
Geer Lou: Shanghai Biotree Biotech Co. Ltd
Junmin Pan: Qingdao National Laboratory for Marine Science and Technology
Muqing Cao: Shanghai Jiao Tong University School of Medicine

Nature Communications, 2022, vol. 13, issue 1, 1-14

Abstract: Abstract The transition zone (TZ) of the cilium/flagellum serves as a diffusion barrier that controls the entry/exit of ciliary proteins. Mutations of the TZ proteins disrupt barrier function and lead to multiple human diseases. However, the systematic regulation of ciliary composition and signaling-related processes by different TZ proteins is not completely understood. Here, we reveal that loss of TCTN1 in Chlamydomonas reinhardtii disrupts the assembly of wedge-shaped structures in the TZ. Proteomic analysis of cilia from WT and three TZ mutants, tctn1, cep290, and nphp4, shows a unique role of each TZ subunit in the regulation of ciliary composition, explaining the phenotypic diversity of different TZ mutants. Interestingly, we find that defects in the TZ impair the formation and biological activity of ciliary ectosomes. Collectively, our findings provide systematic insights into the regulation of ciliary composition by TZ proteins and reveal a link between the TZ and ciliary ectosomes.

Date: 2022
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DOI: 10.1038/s41467-022-31751-0

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