Single-cell transcriptome and translatome dual-omics reveals potential mechanisms of human oocyte maturation

Wenqi Hu, Haitao Zeng, Yanan Shi, Chuanchuan Zhou, Jiana Huang, Lei Jia, Siqi Xu, Xiaoyu Feng, Yanyan Zeng, Tuanlin Xiong, Wenze Huang, Peng Sun, Yajie Chang, Tingting Li, Cong Fang, Keliang Wu, Lingbo Cai, Wuhua Ni, Yan Li, Zhiyong Yang, Qiangfeng Cliff Zhang, RiCheng Chian, Zijiang Chen, Xiaoyan Liang () and Kehkooi Kee ()
Additional contact information
Wenqi Hu: Tsinghua University
Haitao Zeng: The Sixth Affiliated Hospital of Sun Yat-sen University
Yanan Shi: The Sixth Affiliated Hospital of Sun Yat-sen University
Chuanchuan Zhou: The Sixth Affiliated Hospital of Sun Yat-sen University
Jiana Huang: The Sixth Affiliated Hospital of Sun Yat-sen University
Lei Jia: The Sixth Affiliated Hospital of Sun Yat-sen University
Siqi Xu: Tsinghua University
Xiaoyu Feng: Tsinghua University
Yanyan Zeng: The Sixth Affiliated Hospital of Sun Yat-sen University
Tuanlin Xiong: Tsinghua University
Wenze Huang: Tsinghua University
Peng Sun: The Sixth Affiliated Hospital of Sun Yat-sen University
Yajie Chang: The Sixth Affiliated Hospital of Sun Yat-sen University
Tingting Li: The Sixth Affiliated Hospital of Sun Yat-sen University
Cong Fang: The Sixth Affiliated Hospital of Sun Yat-sen University
Keliang Wu: Shandong University
Lingbo Cai: Nanjing Medical University
Wuhua Ni: The First Affiliated Hospital of Wenzhou Medical University
Yan Li: The First Affiliated Hospital of Wenzhou Medical University
Zhiyong Yang: Shanghai Tenth People’s Hospital of Tongji University
Qiangfeng Cliff Zhang: Tsinghua University
RiCheng Chian: Shanghai Tenth People’s Hospital of Tongji University
Zijiang Chen: Shandong University
Xiaoyan Liang: The Sixth Affiliated Hospital of Sun Yat-sen University
Kehkooi Kee: Tsinghua University

Nature Communications, 2022, vol. 13, issue 1, 1-17

Abstract: Abstract The combined use of transcriptome and translatome as indicators of gene expression profiles is usually more accurate than the use of transcriptomes alone, especially in cell types governed by translational regulation, such as mammalian oocytes. Here, we developed a dual-omics methodology that includes both transcriptome and translatome sequencing (T&T-seq) of single-cell oocyte samples, and we used it to characterize the transcriptomes and translatomes during mouse and human oocyte maturation. T&T-seq analysis revealed distinct translational expression patterns between mouse and human oocytes and delineated a sequential gene expression regulation from the cytoplasm to the nucleus during human oocyte maturation. By these means, we also identified a functional role of OOSP2 inducing factor in human oocyte maturation, as human recombinant OOSP2 induced in vitro maturation of human oocytes, which was blocked by anti-OOSP2. Single-oocyte T&T-seq analyses further elucidated that OOSP2 induces specific signaling pathways, including small GTPases, through translational regulation.

Date: 2022
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DOI: 10.1038/s41467-022-32791-2

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