Thermophoretic glycan profiling of extracellular vesicles for triple-negative breast cancer management

Yike Li, Shaohua Zhang, Chao Liu (), Jinqi Deng, Fei Tian, Qiang Feng, Lili Qin, Lixiao Bai, Ting Fu, Liqin Zhang (), Yuguang Wang () and Jiashu Sun ()
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Yike Li: CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology
Shaohua Zhang: the Fifth Medical Center of PLA General Hospital
Chao Liu: CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology
Jinqi Deng: CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology
Fei Tian: CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology
Qiang Feng: CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology
Lili Qin: the Fifth Medical Center of PLA General Hospital
Lixiao Bai: the Fifth Medical Center of PLA General Hospital
Ting Fu: Chinese Academy of Sciences, Key Laboratory of Zhejiang Province for Aptamers and Theranostics
Liqin Zhang: School of Pharmaceutical Sciences, Peking University
Yuguang Wang: Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices
Jiashu Sun: CAS Key Laboratory of Standardization and Measurement for Nanotechnology, National Center for Nanoscience and Technology

Nature Communications, 2024, vol. 15, issue 1, 1-15

Abstract: Abstract Triple-negative breast cancer (TNBC) is a highly metastatic and heterogeneous type of breast cancer with poor outcomes. Precise, non-invasive methods for diagnosis, monitoring and prognosis of TNBC are particularly challenging due to a paucity of TNBC biomarkers. Glycans on extracellular vesicles (EVs) hold the promise as valuable biomarkers, but conventional methods for glycan analysis are not feasible in clinical practice. Here, we report that a lectin-based thermophoretic assay (EVLET) streamlines vibrating membrane filtration (VMF) and thermophoretic amplification, allowing for rapid, sensitive, selective and cost-effective EV glycan profiling in TNBC plasma. A pilot cohort study shows that the EV glycan signature reaches 91% accuracy for TNBC detection and 96% accuracy for longitudinal monitoring of TNBC therapeutic response. Moreover, we demonstrate the potential of EV glycan signature for predicting TNBC progression. Our EVLET system lays the foundation for non-invasive cancer management by EV glycans.

Date: 2024
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DOI: 10.1038/s41467-024-46557-5

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