Purines enrich root-associated Pseudomonas and improve wild soybean growth under salt stress

Yanfen Zheng, Xuwen Cao, Yanan Zhou, Siqi Ma, Youqiang Wang, Zhe Li, Donglin Zhao, Yanzhe Yang, Han Zhang, Chen Meng, Zhihong Xie, Xiaona Sui, Kangwen Xu, Yiqiang Li and Cheng-Sheng Zhang ()
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Yanfen Zheng: Tobacco Research Institute of Chinese Academy of Agricultural Sciences
Xuwen Cao: Shandong University
Yanan Zhou: Tobacco Research Institute of Chinese Academy of Agricultural Sciences
Siqi Ma: Tobacco Research Institute of Chinese Academy of Agricultural Sciences
Youqiang Wang: Tobacco Research Institute of Chinese Academy of Agricultural Sciences
Zhe Li: Tobacco Research Institute of Chinese Academy of Agricultural Sciences
Donglin Zhao: Tobacco Research Institute of Chinese Academy of Agricultural Sciences
Yanzhe Yang: Tobacco Research Institute of Chinese Academy of Agricultural Sciences
Han Zhang: Tobacco Research Institute of Chinese Academy of Agricultural Sciences
Chen Meng: Tobacco Research Institute of Chinese Academy of Agricultural Sciences
Zhihong Xie: College of Resources and Environment of Shandong Agricultural University
Xiaona Sui: Tobacco Research Institute of Chinese Academy of Agricultural Sciences
Kangwen Xu: Tobacco Research Institute of Chinese Academy of Agricultural Sciences
Yiqiang Li: Tobacco Research Institute of Chinese Academy of Agricultural Sciences
Cheng-Sheng Zhang: Tobacco Research Institute of Chinese Academy of Agricultural Sciences

Nature Communications, 2024, vol. 15, issue 1, 1-14

Abstract: Abstract The root-associated microbiota plays an important role in the response to environmental stress. However, the underlying mechanisms controlling the interaction between salt-stressed plants and microbiota are poorly understood. Here, by focusing on a salt-tolerant plant wild soybean (Glycine soja), we demonstrate that highly conserved microbes dominated by Pseudomonas are enriched in the root and rhizosphere microbiota of salt-stressed plant. Two corresponding Pseudomonas isolates are confirmed to enhance the salt tolerance of wild soybean. Shotgun metagenomic and metatranscriptomic sequencing reveal that motility-associated genes, mainly chemotaxis and flagellar assembly, are significantly enriched and expressed in salt-treated samples. We further find that roots of salt stressed plants secreted purines, especially xanthine, which induce motility of the Pseudomonas isolates. Moreover, exogenous application for xanthine to non-stressed plants results in Pseudomonas enrichment, reproducing the microbiota shift in salt-stressed root. Finally, Pseudomonas mutant analysis shows that the motility related gene cheW is required for chemotaxis toward xanthine and for enhancing plant salt tolerance. Our study proposes that wild soybean recruits beneficial Pseudomonas species by exudating key metabolites (i.e., purine) against salt stress.

Date: 2024
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DOI: 10.1038/s41467-024-47773-9

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