A quantitative gibberellin signaling biosensor reveals a role for gibberellins in internode specification at the shoot apical meristem

Bihai Shi, Amelia Felipo-Benavent, Guillaume Cerutti, Carlos Galvan-Ampudia, Lucas Jilli, Geraldine Brunoud, Jérome Mutterer, Elody Vallet, Lali Sakvarelidze-Achard, Jean-Michel Davière, Alejandro Navarro-Galiano, Ankit Walia, Shani Lazary, Jonathan Legrand, Roy Weinstain, Alexander M. Jones, Salomé Prat, Patrick Achard () and Teva Vernoux ()
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Bihai Shi: South China Agricultural University, Guangdong Laboratory for Lingnan Modern Agriculture
Amelia Felipo-Benavent: CNRS, Université de Strasbourg
Guillaume Cerutti: Univ Lyon, ENS de Lyon, CNRS, INRAE, INRIA
Carlos Galvan-Ampudia: Univ Lyon, ENS de Lyon, CNRS, INRAE, INRIA
Lucas Jilli: CNRS, Université de Strasbourg
Geraldine Brunoud: Univ Lyon, ENS de Lyon, CNRS, INRAE, INRIA
Jérome Mutterer: CNRS, Université de Strasbourg
Elody Vallet: CNRS, Université de Strasbourg
Lali Sakvarelidze-Achard: CNRS, Université de Strasbourg
Jean-Michel Davière: CNRS, Université de Strasbourg
Alejandro Navarro-Galiano: 08193 Cerdanyola
Ankit Walia: Cambridge University
Shani Lazary: Tel Aviv University
Jonathan Legrand: Univ Lyon, ENS de Lyon, CNRS, INRAE, INRIA
Roy Weinstain: Tel Aviv University
Alexander M. Jones: Cambridge University
Salomé Prat: 08193 Cerdanyola
Patrick Achard: CNRS, Université de Strasbourg
Teva Vernoux: Univ Lyon, ENS de Lyon, CNRS, INRAE, INRIA

Nature Communications, 2024, vol. 15, issue 1, 1-18

Abstract: Abstract Growth at the shoot apical meristem (SAM) is essential for shoot architecture construction. The phytohormones gibberellins (GA) play a pivotal role in coordinating plant growth, but their role in the SAM remains mostly unknown. Here, we developed a ratiometric GA signaling biosensor by engineering one of the DELLA proteins, to suppress its master regulatory function in GA transcriptional responses while preserving its degradation upon GA sensing. We demonstrate that this degradation-based biosensor accurately reports on cellular changes in GA levels and perception during development. We used this biosensor to map GA signaling activity in the SAM. We show that high GA signaling is found primarily in cells located between organ primordia that are the precursors of internodes. By gain- and loss-of-function approaches, we further demonstrate that GAs regulate cell division plane orientation to establish the typical cellular organization of internodes, thus contributing to internode specification in the SAM.

Date: 2024
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DOI: 10.1038/s41467-024-48116-4

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