Metabolism of l-arabinose converges with virulence regulation to promote enteric pathogen fitness

Curtis Cottam, Rhys T. White, Lauren C. Beck, Christopher J. Stewart, Scott A. Beatson, Elisabeth C. Lowe, Rhys Grinter and James P. R. Connolly ()
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Curtis Cottam: Newcastle University
Rhys T. White: Institute of Environmental Science and Research
Lauren C. Beck: Newcastle University
Christopher J. Stewart: Newcastle University
Scott A. Beatson: The University of Queensland
Elisabeth C. Lowe: Newcastle University
Rhys Grinter: Monash University
James P. R. Connolly: Newcastle University

Nature Communications, 2024, vol. 15, issue 1, 1-14

Abstract: Abstract Virulence and metabolism are often interlinked to control the expression of essential colonisation factors in response to host-associated signals. Here, we identified an uncharacterised transporter of the dietary monosaccharide ʟ-arabinose that is widely encoded by the zoonotic pathogen enterohaemorrhagic Escherichia coli (EHEC), required for full competitive fitness in the mouse gut and highly expressed during human infection. Discovery of this transporter suggested that EHEC strains have an enhanced ability to scavenge ʟ-arabinose and therefore prompted us to investigate the impact of this nutrient on pathogenesis. Accordingly, we discovered that ʟ-arabinose enhances expression of the EHEC type 3 secretion system, increasing its ability to colonise host cells, and that the underlying mechanism is dependent on products of its catabolism rather than the sensing of ʟ-arabinose as a signal. Furthermore, using the murine pathogen Citrobacter rodentium, we show that ʟ-arabinose metabolism provides a fitness benefit during infection via virulence factor regulation, as opposed to supporting pathogen growth. Finally, we show that this mechanism is not restricted to ʟ-arabinose and extends to other pentose sugars with a similar metabolic fate. This work highlights the importance integrating central metabolism with virulence regulation in order to maximise competitive fitness of enteric pathogens within the host-niche.

Date: 2024
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DOI: 10.1038/s41467-024-48933-7

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