High-speed optical imaging with sCMOS pixel reassignment

Biagio Mandracchia, Corey Zheng, Suraj Rajendran, Wenhao Liu, Parvin Forghani, Chunhui Xu and Shu Jia ()
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Biagio Mandracchia: Georgia Institute of Technology and Emory University
Corey Zheng: Georgia Institute of Technology and Emory University
Suraj Rajendran: Georgia Institute of Technology and Emory University
Wenhao Liu: Georgia Institute of Technology and Emory University
Parvin Forghani: Emory University
Chunhui Xu: Emory University
Shu Jia: Georgia Institute of Technology and Emory University

Nature Communications, 2024, vol. 15, issue 1, 1-12

Abstract: Abstract Fluorescence microscopy has undergone rapid advancements, offering unprecedented visualization of biological events and shedding light on the intricate mechanisms governing living organisms. However, the exploration of rapid biological dynamics still poses a significant challenge due to the limitations of current digital camera architectures and the inherent compromise between imaging speed and other capabilities. Here, we introduce sHAPR, a high-speed acquisition technique that leverages the operating principles of sCMOS cameras to capture fast cellular and subcellular processes. sHAPR harnesses custom fiber optics to convert microscopy images into one-dimensional recordings, enabling acquisition at the maximum camera readout rate, typically between 25 and 250 kHz. We have demonstrated the utility of sHAPR with a variety of phantom and dynamic systems, including high-throughput flow cytometry, cardiomyocyte contraction, and neuronal calcium waves, using a standard epi-fluorescence microscope. sHAPR is highly adaptable and can be integrated into existing microscopy systems without requiring extensive platform modifications. This method pushes the boundaries of current fluorescence imaging capabilities, opening up new avenues for investigating high-speed biological phenomena.

Date: 2024
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DOI: 10.1038/s41467-024-48987-7

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