A fluorescent perilipin 2 knock-in mouse model reveals a high abundance of lipid droplets in the developing and adult brain

Sofia Madsen, Ana C. Delgado, Christelle Cadilhac, Vanille Maillard, Fabrice Battiston, Carla Marie Igelbüscher, Simon De Neck, Elia Magrinelli, Denis Jabaudon, Ludovic Telley, Fiona Doetsch and Marlen Knobloch ()
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Sofia Madsen: University of Lausanne
Ana C. Delgado: University of Basel
Christelle Cadilhac: University of Geneva
Vanille Maillard: University of Lausanne
Fabrice Battiston: University of Lausanne
Carla Marie Igelbüscher: University of Lausanne
Simon De Neck: University of Zurich
Elia Magrinelli: University of Lausanne
Denis Jabaudon: University of Geneva
Ludovic Telley: University of Lausanne
Fiona Doetsch: University of Basel
Marlen Knobloch: University of Lausanne

Nature Communications, 2024, vol. 15, issue 1, 1-20

Abstract: Abstract Lipid droplets (LDs) are dynamic lipid storage organelles. They are tightly linked to metabolism and can exert protective functions, making them important players in health and disease. Most LD studies in vivo rely on staining methods, providing only a snapshot. We therefore developed a LD-reporter mouse by labelling the endogenous LD coat protein perilipin 2 (PLIN2) with tdTomato, enabling staining-free fluorescent LD visualisation in living and fixed tissues and cells. Here we validate this model under standard and high-fat diet conditions and demonstrate that LDs are highly abundant in various cell types in the healthy brain, including neurons, astrocytes, ependymal cells, neural stem/progenitor cells and microglia. Furthermore, we also show that LDs are abundant during brain development and can be visualized using live imaging of embryonic slices. Taken together, our tdTom-Plin2 mouse serves as a novel tool to study LDs and their dynamics under both physiological and diseased conditions in all tissues expressing Plin2.

Date: 2024
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DOI: 10.1038/s41467-024-49449-w

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