Natural variation in BnaA07.MKK9 confers resistance to Sclerotinia stem rot in oilseed rape

Li Lin, Xingrui Zhang, Jialin Fan, Jiawei Li, Sichao Ren, Xin Gu, Panpan Li, Meiling Xu, Jingyi Xu, Wenjing Lei, Dongxiao Liu, Qinfu Sun, Guangqin Cai, Qing-Yong Yang, Youping Wang () and Jian Wu ()
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Li Lin: Yangzhou University
Xingrui Zhang: Yangzhou University
Jialin Fan: Yangzhou University
Jiawei Li: Huazhong Agricultural University
Sichao Ren: Yangzhou University
Xin Gu: Yangzhou University
Panpan Li: Yangzhou University
Meiling Xu: Yangzhou University
Jingyi Xu: Yangzhou University
Wenjing Lei: Yangzhou University
Dongxiao Liu: Yangzhou University
Qinfu Sun: Yangzhou University
Guangqin Cai: Chinese Academy of Agricultural Sciences
Qing-Yong Yang: Huazhong Agricultural University
Youping Wang: Yangzhou University
Jian Wu: Yangzhou University

Nature Communications, 2024, vol. 15, issue 1, 1-18

Abstract: Abstract Sclerotinia stem rot (SSR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating diseases for several major oil-producing crops. Despite its impact, the genetic basis of SSR resistance in plants remains poorly understood. Here, through a genome-wide association study, we identify a key gene, BnaA07. MKK9, that encodes a mitogen-activated protein kinase kinase that confers SSR resistance in oilseed rape. Our functional analyses reveal that BnaA07.MKK9 interacts with BnaC03.MPK3 and BnaC03.MPK6 and phosphorylates them at the TEY activation motif, triggering a signaling cascade that initiates biosynthesis of ethylene, camalexin, and indole glucosinolates, and promotes accumulation of H2O2 and the hypersensitive response, ultimately conferring resistance. Furthermore, variations in the coding sequence of BnaA07.MKK9 alter its kinase activity and improve SSR resistance by ~30% in cultivars carrying the advantageous haplotype. These findings enhance our understanding of SSR resistance and may help engineer novel diversity for future breeding of oilseed rape.

Date: 2024
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DOI: 10.1038/s41467-024-49504-6

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