Fibre-specific mitochondrial protein abundance is linked to resting and post-training mitochondrial content in the muscle of men

Elizabeth G. Reisman, Javier Botella, Cheng Huang, Ralf B. Schittenhelm, David A. Stroud, Cesare Granata, Owala S. Chandrasiri, Georg Ramm, Viola Oorschot, Nikeisha J. Caruana () and David J. Bishop ()
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Elizabeth G. Reisman: Victoria University
Javier Botella: Victoria University
Cheng Huang: Monash University
Ralf B. Schittenhelm: Monash University
David A. Stroud: The University of Melbourne
Cesare Granata: Victoria University
Owala S. Chandrasiri: Victoria University
Georg Ramm: Monash University
Viola Oorschot: Monash University
Nikeisha J. Caruana: Victoria University
David J. Bishop: Victoria University

Nature Communications, 2024, vol. 15, issue 1, 1-19

Abstract: Abstract Analyses of mitochondrial adaptations in human skeletal muscle have mostly used whole-muscle samples, where results may be confounded by the presence of a mixture of type I and II muscle fibres. Using our adapted mass spectrometry-based proteomics workflow, we provide insights into fibre-specific mitochondrial differences in the human skeletal muscle of men before and after training. Our findings challenge previous conclusions regarding the extent of fibre-type-specific remodelling of the mitochondrial proteome and suggest that most baseline differences in mitochondrial protein abundances between fibre types reported by us, and others, might be due to differences in total mitochondrial content or a consequence of adaptations to habitual physical activity (or inactivity). Most training-induced changes in different mitochondrial functional groups, in both fibre types, were no longer significant in our study when normalised to changes in markers of mitochondrial content.

Date: 2024
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DOI: 10.1038/s41467-024-50632-2

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