Single-nucleosome imaging unveils that condensins and nucleosome–nucleosome interactions differentially constrain chromatin to organize mitotic chromosomes

Kayo Hibino, Yuji Sakai, Sachiko Tamura, Masatoshi Takagi, Katsuhiko Minami, Toyoaki Natsume, Masa A. Shimazoe, Masato T. Kanemaki, Naoko Imamoto and Kazuhiro Maeshima ()
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Kayo Hibino: National Institute of Genetics
Yuji Sakai: Yokohama City University
Sachiko Tamura: National Institute of Genetics
Masatoshi Takagi: RIKEN Cluster for Pioneering Research
Katsuhiko Minami: National Institute of Genetics
Toyoaki Natsume: SOKENDAI
Masa A. Shimazoe: National Institute of Genetics
Masato T. Kanemaki: SOKENDAI
Naoko Imamoto: RIKEN Cluster for Pioneering Research
Kazuhiro Maeshima: National Institute of Genetics

Nature Communications, 2024, vol. 15, issue 1, 1-18

Abstract: Abstract For accurate mitotic cell division, replicated chromatin must be assembled into chromosomes and faithfully segregated into daughter cells. While protein factors like condensin play key roles in this process, it is unclear how chromosome assembly proceeds as molecular events of nucleosomes in living cells and how condensins act on nucleosomes to organize chromosomes. To approach these questions, we investigate nucleosome behavior during mitosis of living human cells using single-nucleosome tracking, combined with rapid-protein depletion technology and computational modeling. Our results show that local nucleosome motion becomes increasingly constrained during mitotic chromosome assembly, which is functionally distinct from condensed apoptotic chromatin. Condensins act as molecular crosslinkers, locally constraining nucleosomes to organize chromosomes. Additionally, nucleosome-nucleosome interactions via histone tails constrain and compact whole chromosomes. Our findings elucidate the physical nature of the chromosome assembly process during mitosis.

Date: 2024
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DOI: 10.1038/s41467-024-51454-y

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