In situ structural determination of cyanobacterial phycobilisome–PSII supercomplex by STAgSPA strategy

Xing Zhang (), Yanan Xiao, Xin You, Shan Sun and Sen-Fang Sui ()
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Xing Zhang: Tsinghua University
Yanan Xiao: Southern University of Science and Technology
Xin You: Tsinghua University
Shan Sun: Tsinghua University
Sen-Fang Sui: Southern University of Science and Technology

Nature Communications, 2024, vol. 15, issue 1, 1-11

Abstract: Abstract Photosynthesis converting solar energy to chemical energy is one of the most important chemical reactions on earth. In cyanobacteria, light energy is captured by antenna system phycobilisomes (PBSs) and transferred to photosynthetic reaction centers of photosystem II (PSII) and photosystem I (PSI). While most of the protein complexes involved in photosynthesis have been characterized by in vitro structural analyses, how these protein complexes function together in vivo is not well understood. Here we implemented STAgSPA, an in situ structural analysis strategy, to solve the native structure of PBS–PSII supercomplex from the cyanobacteria Arthrospira sp. FACHB439 at resolution of ~3.5 Å. The structure reveals coupling details among adjacent PBSs and PSII dimers, and the collaborative energy transfer mechanism mediated by multiple super-PBS in cyanobacteria. Our results provide insights into the diversity of photosynthesis-related systems between prokaryotic cyanobacteria and eukaryotic red algae but are also a methodological demonstration for high-resolution structural analysis in cellular or tissue samples.

Date: 2024
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DOI: 10.1038/s41467-024-51460-0

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