Endomembrane trafficking driven by microtubule growth regulates stomatal movement in Arabidopsis

Hua Zhong, Shuwei Wang, Yaohui Huang, Xiankui Cui, Xuening Ding, Lei Zhu, Ming Yuan and Ying Fu ()
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Hua Zhong: China Agricultural University
Shuwei Wang: China Agricultural University
Yaohui Huang: China Agricultural University
Xiankui Cui: China Agricultural University
Xuening Ding: China Agricultural University
Lei Zhu: China Agricultural University
Ming Yuan: China Agricultural University
Ying Fu: China Agricultural University

Nature Communications, 2024, vol. 15, issue 1, 1-15

Abstract: Abstract Microtubule-based vesicle trafficking usually relies upon kinesin and dynein motors and few reports describe microtubule polymerisation driving directional vesicle trafficking. Here we show that Arabidopsis END BINDING1b (EB1b), a microtubule plus-end binding protein, directly interacts with SYP121, a SNARE protein that mediates the trafficking of the K+ channel KAT1 and its distribution to the plasma membrane (PM) in Arabidopsis guard cells. Knockout of AtEB1b and its homologous proteins results in a modest but significant change in the distribution of KAT1 and SYP121 in guard cells and consequently delays light-induced stomatal opening. Live-cell imaging reveals that a portion of SYP121-associated endomembrane compartments co-localise with AtEB1b at the growing ends of microtubules, trafficking along with the growth of microtubules for targeting to the PM. Our study reveals a mechanism of vesicle trafficking driven by microtubule growth, which is involved in the redistribution of PM proteins to modulate guard cell movement.

Date: 2024
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DOI: 10.1038/s41467-024-52338-x

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