Mettl1-dependent m7G tRNA modification is essential for maintaining spermatogenesis and fertility in Drosophila melanogaster

Shunya Kaneko, Keita Miyoshi, Kotaro Tomuro, Makoto Terauchi, Ryoya Tanaka, Shu Kondo, Naoki Tani, Kei-Ichiro Ishiguro, Atsushi Toyoda, Azusa Kamikouchi, Hideki Noguchi, Shintaro Iwasaki and Kuniaki Saito ()
Additional contact information
Shunya Kaneko: Research Organization of Information and Systems (ROIS)
Keita Miyoshi: Research Organization of Information and Systems (ROIS)
Kotaro Tomuro: RIKEN Cluster for Pioneering Research
Makoto Terauchi: Research Organization of Information and Systems (ROIS)
Ryoya Tanaka: Nagoya University
Shu Kondo: Tokyo University of Science
Naoki Tani: Kumamoto University
Kei-Ichiro Ishiguro: Kumamoto University
Atsushi Toyoda: Research Organization of Information and Systems (ROIS)
Azusa Kamikouchi: Nagoya University
Hideki Noguchi: Research Organization of Information and Systems (ROIS)
Shintaro Iwasaki: RIKEN Cluster for Pioneering Research
Kuniaki Saito: Research Organization of Information and Systems (ROIS)

Nature Communications, 2024, vol. 15, issue 1, 1-17

Abstract: Abstract Modification of guanosine to N7-methylguanosine (m7G) in the variable loop region of tRNA is catalyzed by the METTL1/WDR4 heterodimer and stabilizes target tRNA. Here, we reveal essential functions of Mettl1 in Drosophila fertility. Knockout of Mettl1 (Mettl1-KO) causes no major effect on the development of non-gonadal tissues, but abolishes the production of elongated spermatids and mature sperm, which is fully rescued by expression of a Mettl1-transgene, but not a catalytic-dead Mettl1 transgene. This demonstrates that Mettl1-dependent m7G is required for spermatogenesis. Mettl1-KO results in a loss of m7G modification on a subset of tRNAs and decreased tRNA abundance. Ribosome profiling shows that Mettl1-KO led to ribosomes stalling at codons decoded by tRNAs that were reduced in abundance. Mettl1-KO also significantly reduces the translation efficiency of genes involved in elongated spermatid formation and sperm stability. Germ cell-specific expression of Mettl1 rescues disrupted m7G tRNA modification and tRNA abundance in Mettl1-KO testes but not in non-gonadal tissues. Ribosome stalling is much less detectable in non-gonadal tissues than in Mettl1-KO testes. These findings reveal a developmental role for m7G tRNA modification and indicate that m7G modification-dependent tRNA abundance differs among tissues.

Date: 2024
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DOI: 10.1038/s41467-024-52389-0

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