High-parametric protein maps reveal the spatial organization in early-developing human lung

Sanem Sariyar, Alexandros Sountoulidis, Jan Niklas Hansen, Sergio Marco Salas, Mariya Mardamshina, Anna Martinez Casals, Frederic Ballllosera Navarro, Zaneta Andrusivova, Xiaofei Li, Paulo Czarnewski, Joakim Lundeberg, Sten Linnarsson, Mats Nilsson, Erik Sundström, Christos Samakovlis, Emma Lundberg () and Burcu Ayoglu ()
Additional contact information
Sanem Sariyar: Science for Life Laboratory
Alexandros Sountoulidis: Science for Life Laboratory
Jan Niklas Hansen: Science for Life Laboratory
Sergio Marco Salas: Science for Life Laboratory
Mariya Mardamshina: Science for Life Laboratory
Anna Martinez Casals: Science for Life Laboratory
Frederic Ballllosera Navarro: Science for Life Laboratory
Zaneta Andrusivova: Science for Life Laboratory
Xiaofei Li: Karolinska Institutet
Paulo Czarnewski: Science for Life Laboratory
Joakim Lundeberg: Science for Life Laboratory
Sten Linnarsson: Karolinska Institute
Mats Nilsson: Science for Life Laboratory
Erik Sundström: Karolinska Institutet
Christos Samakovlis: Science for Life Laboratory
Emma Lundberg: Science for Life Laboratory
Burcu Ayoglu: Science for Life Laboratory

Nature Communications, 2024, vol. 15, issue 1, 1-19

Abstract: Abstract The respiratory system, including the lungs, is essential for terrestrial life. While recent research has advanced our understanding of lung development, much still relies on animal models and transcriptome analyses. In this study conducted within the Human Developmental Cell Atlas (HDCA) initiative, we describe the protein-level spatiotemporal organization of the lung during the first trimester of human gestation. Using high-parametric tissue imaging with a 30-plex antibody panel, we analyzed human lung samples from 6 to 13 post-conception weeks, generating data from over 2 million cells across five developmental timepoints. We present a resource detailing spatially resolved cell type composition of the developing human lung, including proliferative states, immune cell patterns, spatial arrangement traits, and their temporal evolution. This represents an extensive single-cell resolved protein-level examination of the developing human lung and provides a valuable resource for further research into the developmental roots of human respiratory health and disease.

Date: 2024
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DOI: 10.1038/s41467-024-53752-x

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