Mina53 demethylates histone H4 arginine 3 asymmetric dimethylation to regulate neural stem/progenitor cell identity

Lixiao Zhou, Xingsen Zhao, Jie Sun, Kun Zou, Xiaoli Huang, Liyang Yu, Mingxuan Wu, Yong Wang, Xuekun Li () and Wen Yi ()
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Lixiao Zhou: Zhejiang University
Xingsen Zhao: Xianghu Laboratory
Jie Sun: Zhejiang University
Kun Zou: Westlake University
Xiaoli Huang: Zhejiang University
Liyang Yu: Zhejiang University
Mingxuan Wu: Westlake University
Yong Wang: Zhejiang University
Xuekun Li: Zhejiang University
Wen Yi: Zhejiang University

Nature Communications, 2024, vol. 15, issue 1, 1-14

Abstract: Abstract Arginine methylation of histones plays a critical role in regulating gene expression. The writers (methyltransferases) and readers of methylarginine marks are well-known, but the erasers–arginine demethylases–remain mysterious. Here we identify Myc-induced nuclear antigen 53 (Mina53), a jumonji C domain containing protein, as an arginine demethylase for removing asymmetric di-methylation at arginine 3 of histone H4 (H4R3me2a). Using a photoaffinity capture method, we first identify Mina53 as an interactor of H4R3me2a. Biochemical assays in vitro and in cells characterize the arginine demethylation activity of Mina53. Molecular dynamics simulations provide further atomic-level evidence that Mina53 acts on H4R3me2a. In a transgenic mouse model, specific Mina53 deletion in neural stem/progenitor cells prevents H4R3me2a demethylation at distinct genes clusters, dysregulating genes important for neural stem/progenitor cell proliferation and differentiation, and consequently impairing the cognitive function of mice. Collectively, we identify Mina53 as a bona fide H4R3me2a eraser, expanding the understanding of epigenetic gene regulation.

Date: 2024
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DOI: 10.1038/s41467-024-54680-6

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