Spindle-localized F-actin regulates polar MTOC organization and the fidelity of meiotic spindle formation

Edgar J. Soto-Moreno, Nourhan N. Ali, Florian Küllmer, Veselin Nasufovic, Michaela Frolikova, Olga Tepla, Jaromir Masata, Dirk Trauner, Amanda A. Patterson, Hans-Dieter Arndt, Katerina Komrskova, Magdalena Zernicka-Goetz, David M. Glover and Ahmed Z. Balboula ()
Additional contact information
Edgar J. Soto-Moreno: University of Missouri
Nourhan N. Ali: University of Missouri
Florian Küllmer: Institut für Organische Chemie und Makromolekulare Chemie
Veselin Nasufovic: Institut für Organische Chemie und Makromolekulare Chemie
Michaela Frolikova: Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV
Olga Tepla: Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV
Jaromir Masata: Charles University and General University Hospital in Prague
Dirk Trauner: University of Pennsylvania
Amanda A. Patterson: University of Missouri
Hans-Dieter Arndt: Institut für Organische Chemie und Makromolekulare Chemie
Katerina Komrskova: Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV
Magdalena Zernicka-Goetz: California Institute of Technology
David M. Glover: California Institute of Technology
Ahmed Z. Balboula: University of Missouri

Nature Communications, 2025, vol. 16, issue 1, 1-20

Abstract: Abstract Mammalian oocytes are notoriously prone to chromosome segregation errors leading to aneuploidy. The spindle provides the machinery for accurate chromosome segregation during cell division. Mammalian oocytes lack centrioles and, therefore, mouse meiotic spindle relies on the organization of numerous acentriolar microtubule organizing centers into two poles (polar microtubule organizing centers, pMTOCs). The traditional view is that, in mammalian oocytes, microtubules are the sole cytoskeletal component responsible for regulating pMTOC organization and spindle assembly. We identify a previously unrecognized F-actin pool that surrounds pMTOCs, forming F-actin cage-like structure. We demonstrate that F-actin localization on the spindle depends on unconventional myosins X and VIIb. Selective disruption of spindle-localized F-actin, using myosin X/VIIb knockdown oocytes or photoswitchable Optojasp-1, perturbs pMTOC organization, leading to unfocused spindle poles and chromosome missegregation. Here, we unveil an important function of spindle-localized F-actin in regulating pMTOC organization, a critical process for ensuring the fidelity of meiotic spindle formation and proper chromosome segregation.

Date: 2025
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-025-63586-w Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-63586-w

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-025-63586-w

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().