Enzyme morphinan N-demethylase for more sustainable opiate processing

M. M. Augustin (), J. M. Augustin, J. R. Brock and T. M. Kutchan ()
Additional contact information
M. M. Augustin: Donald Danforth Plant Science Center
J. M. Augustin: Donald Danforth Plant Science Center
J. R. Brock: Donald Danforth Plant Science Center
T. M. Kutchan: Donald Danforth Plant Science Center

Nature Sustainability, 2019, vol. 2, issue 6, 465-474

Abstract: Abstract Naturally occurring opiates, such as morphine and thebaine, are produced in poppy species. Thebaine is converted into painkillers and opiate addiction treatments, the latter requiring a chemical reaction called N-demethylation. Current opiate N-demethylation utilizes noxious reagents, resulting in copious amounts of harmful waste. One way to make opiate production more sustainable is to use enzymes rather than chemicals. Microorganisms provide a rich source of enzymes useful for metabolizing unique compounds in their environment. Therefore, an opium-processing waste stream was probed to identify an organism capable of catalysing opiate N-demethylation. A sludge sample was grown on minimal medium containing thebaine as the sole carbon source, to identify a biocatalyst. This led to the discovery of Thebainfresser, a Methylobacterium that metabolizes opiates by removing the N-methyl group. N-demethylation was induced following growth in minimal medium, a characteristic which led to discovery of the underlying gene MND (morphinan N-demethylase). The enzyme MND was found to be robust and versatile, N-demethylating structurally diverse substrates at varying temperatures and pH levels. In addition, MND tolerated selected organic solvents and maintained activity when immobilized. These properties make it an attractive candidate for further development for pharmaceutical manufacture.

Date: 2019
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41893-019-0302-6 Abstract (text/html)
Access to the full text of the articles in this series is restricted.

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natsus:v:2:y:2019:i:6:d:10.1038_s41893-019-0302-6

Ordering information: This journal article can be ordered from
https://www.nature.com/natsustain/

DOI: 10.1038/s41893-019-0302-6

Access Statistics for this article

Nature Sustainability is currently edited by Monica Contestabile

More articles in Nature Sustainability from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().