Intravascular Immune Surveillance by CXCR6+ NKT Cells Patrolling Liver Sinusoids

Frederic Geissmann, Thomas O Cameron, Stephane Sidobre, Natasha Manlongat, Mitchell Kronenberg, Michael J Briskin, Michael L Dustin and Dan R Littman

PLOS Biology, 2005, vol. 3, issue 4, 1-

Abstract: We examined the in vivo behavior of liver natural killer T cells (NKT cells) by intravital fluorescence microscopic imaging of mice in which a green fluorescent protein cDNA was used to replace the gene encoding the chemokine receptor CXCR6. NKT cells, which account for most CXCR6+ cells in liver, were found to crawl within hepatic sinusoids at 10–20 μm/min and to stop upon T cell antigen receptor activation. CXCR6-deficient mice exhibited a selective and severe reduction of CD1d-reactive NKT cells in the liver and decreased susceptibility to T-cell-dependent hepatitis. CXCL16, the cell surface ligand for CXCR6, is expressed on sinusoidal endothelial cells, and CXCR6 deficiency resulted in reduced survival, but not in altered speed or pattern of patrolling of NKT cells. Thus, NKT cells patrol liver sinusoids to provide intravascular immune surveillance, and CXCR6 contributes to liver-based immune responses by regulating their abundance. Intravital fluorescence microscopy shows that natural killer T cells patrol blood vessels in the liver and stop upon activation, demonstrating their role in liver immune surveillance.

Date: 2005
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pbio00:0030113

DOI: 10.1371/journal.pbio.0030113

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