Optimization of Preparation of Antioxidative Peptides from Pumpkin Seeds Using Response Surface Method

Sanhong Fan, Yanan Hu, Chen Li and Yanrong Liu

PLOS ONE, 2014, vol. 9, issue 3, 1-6

Abstract: Protein isolates of pumpkin (Cucurbita pepo L) seeds were hydrolyzed by acid protease to prepare antioxidative peptides. The hydrolysis conditions were optimized through Box-Behnken experimental design combined with response surface method (RSM). The second-order model, developed for the DPPH radical scavenging activity of pumpkin seed hydrolysates, showed good fit with the experiment data with a high value of coefficient of determination (0.9918). The optimal hydrolysis conditions were determined as follows: hydrolyzing temperature 50°C, pH 2.5, enzyme amount 6000 U/g, substrate concentration 0.05 g/ml and hydrolyzing time 5 h. Under the above conditions, the scavenging activity of DPPH radical was as high as 92.82%.

Date: 2014
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0092335

DOI: 10.1371/journal.pone.0092335

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