Comparison of Multiple Displacement Amplification (MDA) and Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) in Single-Cell Sequencing

Minfeng Chen, Pengfei Song, Dan Zou, Xuesong Hu, Shancen Zhao, Shengjie Gao and Fei Ling

PLOS ONE, 2014, vol. 9, issue 12, 1-12

Abstract: Single-cell sequencing promotes our understanding of the heterogeneity of cellular populations, including the haplotypes and genomic variability among different generation of cells. Whole-genome amplification is crucial to generate sufficient DNA fragments for single-cell sequencing projects. Using sequencing data from single sperms, we quantitatively compare two prevailing amplification methods that extensively applied in single-cell sequencing, multiple displacement amplification (MDA) and multiple annealing and looping-based amplification cycles (MALBAC). Our results show that MALBAC, as a combination of modified MDA and tweaked PCR, has a higher level of uniformity, specificity and reproducibility.

Date: 2014
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0114520

DOI: 10.1371/journal.pone.0114520

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