Quantitative Microplate Assay for Real-Time Nuclease Kinetics

Jonas Eriksson and Ülo Langel

PLOS ONE, 2016, vol. 11, issue 4, 1-9

Abstract: Utilizing the phenomenon of nucleases exposing oligonucleotide phosphate backbones to phosphatases we present a novel quantitative method for kinetics of nuclease catalysis. Inorganic phosphate released from nuclease products by phosphatases could be quantified in real-time by a fluorescent sensor of inorganic phosphate. Two different nucleases were employed, showing the versatility of this assay for multiple turnover label-free nuclease studies.

Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0154099

DOI: 10.1371/journal.pone.0154099

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