Femtosecond-Laser-Pulse Characterization and Optimization for CARS Microscopy

Vincenzo Piazza, Giuseppe de Vito, Elmira Farrokhtakin, Gianni Ciofani and Virgilio Mattoli

PLOS ONE, 2016, vol. 11, issue 5, 1-13

Abstract: We present a simple method and its experimental implementation to determine the pulse durations and linear chirps of the pump-and-probe pulse and the Stokes pulse in a coherent anti-Stokes Raman scattering microscope at sample level without additional autocorrelators. Our approach exploits the delay line, ubiquitous in such microscopes, to perform a convolution of the pump-and-probe and Stokes pulses as a function of their relative delay and it is based on the detection of the photons emitted from an appropriate non-linear sample. The analysis of the non-resonant four-wave-mixing and sum-frequency-generation signals allows for the direct retrieval of the pulse duration on the sample and the linear chirp of each pulse. This knowledge is crucial in maximizing the spectral-resolution and contrast in CARS imaging.

Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0156371

DOI: 10.1371/journal.pone.0156371

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