Thin-Film Transistor-Based Biosensors for Determining Stoichiometry of Biochemical Reactions

Yi-Wen Wang, Ting-Yang Chen, Tsung-Han Yang, Cheng-Chung Chang, Tsung-Lin Yang, Yu-Hwa Lo and Jian-Jang Huang

PLOS ONE, 2016, vol. 11, issue 12, 1-8

Abstract: The enzyme kinetic in a biochemical reaction is critical to scientific research and drug discovery but can hardly be determined experimentally from enzyme assays. In this work, a charge-current transducer (a transistor) is proposed to evaluate the status of biochemical reaction by monitoring the electrical charge changes. Using the malate-aspartate shuttle as an example, a thin-film transistor (TFT)-based biosensor with an extended gold pad is demonstrated to detect the biochemical reaction between NADH and NAD+. The drain current change indicates the status of chemical equilibrium and stoichiometry.

Date: 2016
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0169094

DOI: 10.1371/journal.pone.0169094

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