Impact of LINE-1 hypomethylation on the clinicopathological and molecular features of colorectal cancer patients

Tai-Chuan Kuan, Pei-Ching Lin, Shung-Haur Yang, Chun-Chi Lin, Yuan-Tzu Lan, Hung-Hsin Lin, Wen-Yi Liang, Wei-Shone Chen, Jen-Kou Lin, Jeng-Kai Jiang and Shih-Ching Chang

PLOS ONE, 2018, vol. 13, issue 5, 1-12

Abstract: Recent studies suggest that aberrant DNA methylation might occur early and commonly in colorectal tumorigenesis. In 111 normal subjects, the mean LINE-1 methylation level of peripheral blood was 81.0 ± 5.7%. Of 143 colorectal cancer (CRC) patients, the mean level of LINE-1 methylation was 60.5 ± 12.5%. We defined below 60% as cut-off value of LINE-1 hypomethylation, and 93 cases (65.0%) had LINE-1 hypomethylation in the tumor tissue. LINE-1 hypomethylation was not associated with any other clinical features. There was a trend that LINE-1 hypomethylation tumors were associated with advanced disease, but it did not reach statistical significance. There was no significant association between mutations of 12 genes, MSI-high, EMAST, and LINE-1 hypomethylation level. The median follow-up was 61.2 months. Five-year disease-free survival (DFS) and overall survival curves of patients with LINE-1 hypomethylation tumors were significantly lower than those of patients with normal LINE-1 methylation tumors (p = 0.032 and 0.001, respectively). Multivariate analysis showed that only TNM staging was an independent prognostic factor for CRC patients including DFS and overall survival (OS). LINE-1 did not impact patients’ outcomes in multivariate analysis including DFS and OS. In conclusion, LINE-1 hypomethylation is marginally related to advanced stage CRC and impacts patients’ outcomes in univariate analysis.

Date: 2018
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0197681

DOI: 10.1371/journal.pone.0197681

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