Use of high-content analysis and machine learning to characterize complex microbial samples via morphological analysis

Jennifer Petitte, Michael Doherty, Jacob Ladd, Cassandra L Marin, Samuel Siles, Vanessa Michelou, Amanda Damon, Erin Quattrini Eckert, Xiang Huang and John W Rice

PLOS ONE, 2019, vol. 14, issue 9, 1-15

Abstract: High Content Analysis (HCA) has become a cornerstone of cellular analysis within the drug discovery industry. To expand the capabilities of HCA, we have applied the same analysis methods, validated in numerous mammalian cell models, to microbiology methodology. Image acquisition and analysis of various microbial samples, ranging from pure cultures to culture mixtures containing up to three different bacterial species, were quantified and identified using various machine learning processes. These HCA techniques allow for faster cell enumeration than standard agar-plating methods, identification of “viable but not plate culturable” microbe phenotype, classification of antibiotic treatment effects, and identification of individual microbial strains in mixed cultures. These methods greatly expand the utility of HCA methods and automate tedious and low-throughput standard microbiological methods.

Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0222528

DOI: 10.1371/journal.pone.0222528

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