Sperm kinematic subpopulations of the American crocodile (Crocodylus acutus)

Anthony Valverde, Olivier Castro-Morales, Mónica Madrigal-Valverde, Marlen Camacho, Vinicio Barquero, Carles Soler and Eduardo R S Roldan

PLOS ONE, 2021, vol. 16, issue 3, 1-16

Abstract: There has been very limited use of computer assisted semen analysis (CASA) to evaluate reptile sperm. The aim of this study was to examine sperm kinematic variables in American crocodile (Crocodylus acutus) semen samples and to assess whether sperm subpopulations could be characterized. Eight ejaculates (two ejaculates/male) from four sexually mature captive crocodiles were obtained. An ISAS®v1 CASA-Mot system, with an image acquisition rate of 50 Hz, and ISAS®D4C20 counting chambers were used for sperm analyses. The percentages of motile and progressively motile spermatozoa did not differ among animals (P > 0.05) but there was a significant animal effect with regards to kinematic variables (P

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0248270

DOI: 10.1371/journal.pone.0248270

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