Evaluation of micro-RNA in extracellular vesicles from blood of patients with prostate cancer

Jiyoon Kim, Siwoo Cho, Yonghyun Park, Jiyoul Lee and Jaesung Park

PLOS ONE, 2021, vol. 16, issue 12, 1-11

Abstract: Extracellular vesicles (EVs) contain various types of molecules including micro-RNAs, so isolating EVs can be an effective way to analyze and diagnose diseases. A lot of micro-RNAs have been known in relation to prostate cancer (PCa), and we evaluate miR-21, miR-141, and miR-221 in EVs and compare them with prostate-specific antigen (PSA). EVs were isolated from plasma of 38 patients with prostate cancer and 8 patients with benign prostatic hyperplasia (BPH), using a method that showed the highest recovery of RNA. Isolation of EVs concentrated micro-RNAs, reducing the cycle threshold (Ct) value of RT-qPCR amplification of micro-RNA such as miR-16 by 5.12 and miR-191 by 4.65, compared to the values before EV isolation. Normalization of target micro-RNAs was done using miR-191. For miR-221, the mean expression level of patients with localized PCa was significantly higher than that of the control group, having 33.45 times higher expression than the control group (p

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:plo:pone00:0262017

DOI: 10.1371/journal.pone.0262017

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