Testing Protocols

Mary Beth Flanders Stepans, Susan L. Wilhelm, T. Kim Rodehorst, Derek Smith and Clarann Weinert
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Mary Beth Flanders Stepans: Wyoming State Board of Nursing, Cheyenne, Wyoming
Susan L. Wilhelm: University of Nebraska Medical Center, Scottsbluff, Nebraska
T. Kim Rodehorst: University of Nebraska Medical Center, Scottsbluff, Nebraska
Derek Smith: University of Wyoming, Laramie, Wyoming
Clarann Weinert: Montana State University, Bozeman, Montana, cweinert@montana.edu

Clinical Nursing Research, 2009, vol. 18, issue 1, 6-22

Abstract: The purpose of this study is to evaluate collection, transport, and storage protocols of urine and air allergen samples from infants in a rural setting. Infant urine and aeroallergen samples ( n = 21) are collected in the home setting in rural areas where time and distance to the central laboratory is a consideration. Urine samples are analyzed using ELISA tests after being transported using three different techniques: (a) ambient temperature, (b) dry ice, and (c) packaged on dry ice and shipped commercially. Following initial ELISA testing for levels of cytokines, urine samples are frozen at -70 degrees Celsius for 6 months. Samples are then reanalyzed for levels of cytokines. Dust samples are analyzed for levels of aeroallergens, stored at 4 degrees for 6 months, and reanalyzed. The integrity of samples varies by biomarker, shipment temperature, and storage time, creating a high degree of variability in results.

Keywords: biomarkers; sample collection; rural; infant; respiratory (search for similar items in EconPapers)
Date: 2009
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Persistent link: https://EconPapers.repec.org/RePEc:sae:clnure:v:18:y:2009:i:1:p:6-22

DOI: 10.1177/1054773808327551

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