 Nanoparticles synthesized from extracts of Tetradenia riparia reveal pharmacological propertiesNisha Singh () and
Shakira Shaik ()
No 2805130, Proceedings of International Academic Conferences from International Institute of Social and Economic Sciences Abstract: Plant-mediated synthesis of metallic nanoparticles is a growing area of interest in the fields of green synthesis and nanotechnology. In this study, the synthesis of gold and silver nanoparticles (AuNPs and AgNPs, respectively) in flowers, leaves and stems of Tetradenia riparia using methanol and water was investigated. Following observation by colour change, the nanoparticles were confirmed by ultraviolet (UV) visible spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray (EDX) analysis and Fourier transform infrared spectroscopy (FTIR). UV peaks for AgNPs and AuNPs were recorded at 410 nm and 540 nm, respectively. FTIR analysis indicated that the reducing agents included terpenoids and pyrones which were responsible for reducing and capping the nanoparticles. Furthermore, crude methanol leaf extracts indicated the presence of phenolics and flavonoids (77.9±8.5 and 4.0±0.9 mg g-1 dry weight, respectively). The synthesised nanoparticles were tested for anti-bacterial activity on five pathogenic bacteria. AgNPs were active and showed MIC against E. coli (1.56 µl ml-1), E. faecalis (1.56 µl ml-1), K. pneumonia (1.56 µl ml-1), P. aeruginosa (12.5 µl ml-1) and S. aureus (50 µl ml-1). MIC using AuNPs was as follows: K. pneumonia (1.56 µl ml-1), E. faecalis (1.56 µl ml-1), E. coli (6.25 µl ml-1), P. aeruginosa (12.5 µl ml-1) and S. aureus (50 µl ml-1). The in vitro screening of the nanoparticles showed potential cytotoxic activity against the human breast cancer (MCF7) cell line. Data generated were used to plot a dose-response curve of which the concentration of extract required to kill 50% of MCF7 cell population (IC50) was determined to be 5 and 375 µg ml-1 for AgNPs and AuNPs, respectively, after 96 hrs. Keywords: Cytotoxicity; Iboza; Green synthesis; Nanoparticles; Plant extract (search for similar items in EconPapers) Published in Proceedings of the Proceedings of the 19th International Academic Conference, Florence, Oct 2015, pages 783-783 Downloads: (external link) Related works: Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text Persistent link: https://EconPapers.repec.org/RePEc:sek:iacpro:2805130 Access Statistics for this paper More papers in Proceedings of International Academic Conferences from International Institute of Social and Economic Sciences |
Is your work missing from RePEc? Here is how to contribute.
Questions or problems? Check the EconPapers FAQ or send mail to .
EconPapers is hosted by the
School of Business at Örebro University.