Purification of Protease Enzymes from Wild Poinsettia (Euphorbia Heterophylla) Plant with Ammonium Sulphate

R. Amilia Destryana (), Dyah Ayu Fajarianingtyas, Aryo Wibisono, Yuli Witono, Maria Belgis and Iwan Taruna
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R. Amilia Destryana: Universitas Wiraraja
Dyah Ayu Fajarianingtyas: Universitas Wiraraja
Aryo Wibisono: Universitas Wiraraja
Yuli Witono: Universitas Jember
Maria Belgis: Universitas Jember
Iwan Taruna: Universitas Jember

A chapter in Proceedings of the 2022 Brawijaya International Conference (BIC 2022), 2023, pp 449-456 from Springer

Abstract: Abstract Protease enzymes are one of the essential enzyme groups that have been widely studied and have very high economic value. The study aimed to determine the optimum protease enzyme extraction method from the wild poinsettia (Euphorbia heterophylla) to produce the highest protease enzyme activity. It was a salting-out extraction method with adding ammonium sulphate salt at saturation levels of 50%, 60%, and 70%. The results showed that adding ammonium sulphate at a concentration of 50% is the most effective protease extraction from the latex of wild poinsettia. It showed the highest percentage yield (17.63 ± 4.74%) and the soluble protein content (4.76 ± 0.014%), while the protease enzyme activity was the lowest (2.9 x 103 ± 0.0142 unit/mg). The highest specific protease activity is achieved by extraction with 70% ammonium sulphate, i.e., 1.8 x 104 units/mg, at the saturation level of 50% and 60% ammonium sulphate; the specific activity of the protease wild poinsettia tended to be low. In conclusion, adding ammonium sulphate salt at saturation levels of 50%, 60%, and 70% showed the different characterization of protease enzymes from wild poinsettia plant latex.

Keywords: ammonium sulphate; plant latex; protease activity; protease enzyme (search for similar items in EconPapers)
Date: 2023
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DOI: 10.2991/978-94-6463-140-1_45

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