Assessment of the efficacy of Bacillus thuringiensis and Pseudomonas fluorescens in the biological control of Hypera postica larvae and adults

Wafaa Ayad Al-Nuaimy, Hawraa F Wali and Mouna Akeel Hamed Al-Oebady
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Wafaa Ayad Al-Nuaimy: Department of Biology, College of Science/Al-Muthanna University/ Al Muthanna, Iraq
Hawraa F Wali: Department of Biology, College of Science/Al-Muthanna University/ Al Muthanna, Iraq
Mouna Akeel Hamed Al-Oebady: Department of Biology, College of Science/Al-Muthanna University/ Al Muthanna, Iraq

Technium, 2025, vol. 26, 76-83

Abstract: The research aimed to assess the impact of two bacterial isolates, Bacillus thuringiensis and Pseudomonas fluorescens, on the larvae and adults of Hypera postica in a laboratory setting, utilising three concentrations (1×10², 1×10⁴, and 1×10⁶) CFU / ml for both bacterial kinds under investigation. The study's results indicated that the quantities employed varied in their efficacy in exterminating larvae and adults of the clover weevil H. postica in a laboratory setting. The findings indicated that the rates of mortality escalate with higher concentrations and prolonged feeding durations. The concentration of 1×106 CFU / ml of the B. thuringiensis exhibited the highest mortality rates after 96 hours of treatment, with rates of (75.8%, 73.9%, 69.7%, 68.2%, and 43.5%) for the first, second, third, fourth larval stages, and adults, respectively. In contrast, the lowest mortality rate was observed with the bacterium P. fluorescens at a concentration of 1×102 CFU / ml after 48 hours, yielding rates of (13.5%, 28.4%, 27.7%, 27.0%, and 6.6%) Subsequent to the aforementioned stages.

Date: 2025
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Persistent link: https://EconPapers.repec.org/RePEc:tec:techni:ojs:12383

DOI: 10.47577/technium.v26i.12383

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