ASSESSMENT OF GENETIC DIVERSITY AMONG NIGERIAN SESAME (SESAMUM INDICUM L.) ACCESSIONS USING INTER SIMPLE SEQUENCE REPEATS MARKERS

Felicia Adejoke Durodola (), Amos Oladimeji Adubi and Musibau Adewuyi Azeez
Additional contact information
Felicia Adejoke Durodola: Department of Pure and Applied Biology, Faculty of Pure and Applied Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Amos Oladimeji Adubi: School of Science, Department of Biology, Oyo State College of. Education, Lanlate, Nigeria
Musibau Adewuyi Azeez: Department of Pure and Applied Biology, Faculty of Pure and Applied Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.

Acta Scientifica Malaysia (ASM), 2023, vol. 7, issue 1, 66-74

Abstract: Genetic diversity among 26 sesame accessions in Nigeria was evaluated using inter simple sequence repeat (ISSR) markers. The samples’ genomic DNA was extracted from the leaf, and eleven ISSR markers were used in polymerase chain reaction (PCR) to determine allelic polymorphism, marker effectiveness and genetic relationship of the accessions. Fifty-two loci comprising 885 alleles were amplified by the 11 markers with 82.69% polymorphism. The average number of amplified loci per primer was 4.73 with a polymorphism range of 50-100%. The Principal Coordinate Analysis revealed 86.74% variability with the first three coordinates; PCoA1, PCoA2 and PCoA3 responsible for 20.38%, 13.82%, and 13.11% of the total variation, respectively. The dendrogram separated the accessions into two groups with 9 sub-clusters at a genetic distance of 10 (90% similarity). Jaccard similarity index range was between 0.50 and 0.94. In conclusion, the ISSR markers employed for this study were informative and effective in detecting variations among the accessions. Therefore, there is substantial genetic diversity existing in the twenty-six accessions of sesame which could be used in the selection of suitable parental genotypes for initiation of breeding programme for seed yield and oil quality improvement of sesame plant in the country.

Keywords: DNA; Inter Simple Sequence Repeat (ISSR); polymerase chain reaction (PCR); Dendrogram (search for similar items in EconPapers)
Date: 2023
References: View complete reference list from CitEc
Citations:

Downloads: (external link)
https://actascientificamalaysia.com/archives/ASM/2asm2023/2asm2023-66-74.pdf (application/pdf)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:zib:zbnasm:v:7:y:2023:i:2:p:66-74

DOI: 10.26480/asm.02.2023.66.74

Access Statistics for this article

Acta Scientifica Malaysia (ASM) is currently edited by Dr Gan Sin Yee

More articles in Acta Scientifica Malaysia (ASM) from Zibeline International Publishing
Bibliographic data for series maintained by Zibeline International Publishing ( this e-mail address is bad, please contact ).