Establishment and Optimization of Two-dimensional Electrophoresis System for Spleen Proteome of Sillago sihama Forsskål

Junlin Wang, Xiulan Chen, Zhonghong Hu, Jing Li, Huanying Pang and Yang Huang

Asian Agricultural Research, 2019, vol. 11, issue 03

Abstract: Taking Sillago sihama Forsskål as the research object, through the optimization of the extraction method, hydration and isoelectric focusing, a two-dimensional electrophoresis system was established for spleen proteome of S. sihama Forsskål. The results showed that the twp-step hydration and isoelectric focusing method is better than the hydration and isoelectric focusing integrated method. The protein spots of the spleen of S. sihama Forsskål were detected by two-dimensional electrophoresis mainly at pH 4.0-7.0. The establishment of this technical system will lay a foundation for further research on proteomics of S. sihama Forsskål in the future.

Keywords: Agribusiness (search for similar items in EconPapers)
Date: 2019
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Persistent link: https://EconPapers.repec.org/RePEc:ags:asagre:290297

DOI: 10.22004/ag.econ.290297

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